@article{33382124df5d4fd7af326b4d8dae156a,
title = "Combination Informatic and Experimental Approach for Selecting Scaffold Proteins for Development as Antibody Mimetics",
abstract = "Antibody mimetics are proteins smaller than antibodies that have antigen-binding properties. Here, we used a combination of informatic selection and experimental verification to identify from a public database several candidate scaffold proteins. From those candidates, we selected a protein with high thermal stability, bacterial expression, and mutation tolerance and used molecular engineering and phage display selection techniques to develop the scaffold into an antibody mimetic with binding affinity for galactin-3, a biomarker of cancer and heart disease.",
keywords = "Antibody mimetics, Combinatorial library, Random mutation",
author = "Tomoyuki Ito and Hafumi Nishi and Tomoshi Kameda and Mayu Yoshida and Reito Fukazawa and Sakiya Kawada and Hikaru Nakazawa and Mitsuo Umetsu",
note = "Funding Information: This work was partly supported by a Scientific Research Grant from the Japan Society for the Promotion of Science (M.U., JP20H00315); by grants from the Japan Agency for Medical Research and Development (M.U., JP18am0301016h000) and development of the Key Technologies for the Next-Generation Artificial Intelligence/Robots from the Ministry of Economy, Trade and Industry, Japan (M.U.); and by the research environment of Tohoku University, Division for Interdisciplinary Advanced Research and Education. Publisher Copyright: {\textcopyright} 2021 The Chemical Society of Japan",
year = "2021",
month = nov,
doi = "10.1246/cl.210443",
language = "English",
volume = "50",
pages = "1867--1871",
journal = "Chemistry Letters",
issn = "0366-7022",
publisher = "Chemical Society of Japan",
number = "11",
}
