Combination Informatic and Experimental Approach for Selecting Scaffold Proteins for Development as Antibody Mimetics

Tomoyuki Ito, Hafumi Nishi, Tomoshi Kameda, Mayu Yoshida, Reito Fukazawa, Sakiya Kawada, Hikaru Nakazawa, Mitsuo Umetsu

Research output: Contribution to journalArticlepeer-review

Abstract

Antibody mimetics are proteins smaller than antibodies that have antigen-binding properties. Here, we used a combination of informatic selection and experimental verification to identify from a public database several candidate scaffold proteins. From those candidates, we selected a protein with high thermal stability, bacterial expression, and mutation tolerance and used molecular engineering and phage display selection techniques to develop the scaffold into an antibody mimetic with binding affinity for galactin-3, a biomarker of cancer and heart disease.

Original languageEnglish
Pages (from-to)1867-1871
Number of pages5
JournalChemistry Letters
Volume50
Issue number11
DOIs
Publication statusPublished - 2021 Nov

Keywords

  • Antibody mimetics
  • Combinatorial library
  • Random mutation

ASJC Scopus subject areas

  • Chemistry(all)

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