Colominic acid production from Escherichia coli in a fed-batch culture under the control of ammonium ions using an FIA system

Hiroyuki Honda, Takuo Nakazeko, Koji Ogiso, Yuji Kawase, Nobuhiro Aoki, Mitsuo Kawase, Takeshi Kobayashi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A fed-batch culture was applied for the production of colominic acid from Escherichia coli. It was found that the copper ion, which is a trace element in medium and an essential element in N-acetylneuraminic acid synthase, affected the colominic acid production and the highest production was obtained using 1.6 mg/l CuCl2. Ammonia formed from nitrogen source utilization greatly affected colominic acid production and only slightly affected the cell growth. A flow injection analysis (FIA) system was fabricated and applied to monitor and control the ammonia concentration in the culture broth. It was found that a concentration of 0.3 g/l of ammonia was optimal for colominic acid production where 1.9 g/l was obtained.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalJournal of Fermentation and Bioengineering
Volume83
Issue number1
DOIs
Publication statusPublished - 1997 Jan 1
Externally publishedYes

Keywords

  • Escherichia coli
  • ammonia
  • colominic acid
  • fed-batch culture
  • flow injection analysis

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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