Collagenases in pancreatic islet isolation

Ibrahim Fathi, Masafumi Goto

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Collagenases, essential elements in experimental and clinical islet isolation, have been the subject of numerous investigations over the last three decades in order to standardize and optimize the enzyme blends for pancreatic tissue dissociation. Through these studies, the contribution of separate components of traditional crude clostridial collagenase preparations, including collagenase G (ColG), collagenase H (ColH), neutral proteases, and clostripain, has been largely unraveled. We can, therefore, now redefine the enzyme parameters necessary for optimum digestion and a high yield and quality of islets, coupled with significant advances in the manufacture process. In this chapter, the authors describe the structure of clostridial digestive enzymes with emphasis on collagenase molecular forms, review the progress on activity assays of digestion enzymes, expound on the current understanding of enzyme contribution in relation to extracellular matrix composition in the peri-insular region as well as concerns regarding lot-to-lot variability and enzyme safety, discuss advances in recombinant collagenases and animal-free preparations, and outline the current efforts for a tailored donor-specific approach.

Original languageEnglish
Title of host publicationTransplantation, Bioengineering, and Regeneration of the Endocrine Pancreas
Subtitle of host publicationVolume 1
PublisherElsevier
Pages529-546
Number of pages18
ISBN (Electronic)9780128148334
ISBN (Print)9780128148341
DOIs
Publication statusPublished - 2019 Jan 1

Keywords

  • Basement membrane
  • Clostridium histolyticum
  • Clostripain
  • ColG
  • ColH
  • Collagenase
  • Extracellular matrix
  • Neutral protease
  • Peri-insular region
  • Thermolysin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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