Collagen immobilization onto the surface of artificial hair for improving the tissue adhesion

Koichi Kato, Yoshihito Kikumura, Masaya Yamamoto, Naohide Tomita, Siro Yamada, Yoshito Ikada

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

To improve the bonding ability of artificial hair towards soft tissue, type I atelocollagen was immobilized onto the hair surface. The artificial hair used was made of a poly(ethylene terephthalate) monofilament.Following photo-induced graft polymerization of a hydrophilic monomer onto the surface of artificial hair, collagen was complexed with the graft chains. Poly(acrylic acid) was selected as the polymer tobe grafted onto the artificial hair because this synthetic polymer exhibited the greatest ability to form an interpolymer complex in solution with collagen among the three anionic polymers poly(acrylicacid), poly(2-acrylamido methylpropane sulfonic acid), and sodium poly(styrene sulfonic acid). When the surface of the poly(ethylene terephthalate) film used as a model substrate was grafted with poly(acrylicacid), the surface density of the collagen immobilized by interpolymer complexation was found to increase with increasing surface density of the graft chains. Immobilization of collagen onto the filamentsurface was confirmed by surface analysis with X-ray photoelectron spectroscopy and transmission electron microscopy. It was shown that in vitro degradation of the collagen immobilized onto poly(ethyleneterephthalate) was suppressed by crosslinking the collagen molecules with glutaraldehyde. Cell culture tests revealed that L-cells were attached well to the surface of collagen-immobilized artificial hair.The surface-modified hairs were implanted percutaneously in the scalp of a human volunteer. Neither infection nor rejection of the hair filaments was observed after 1 year of implantation. It was foundthat the number of collagenimmobilized filaments remaining fixed in the scalp after 3 years of implantation was significantly larger than that of untreated filaments. These results indicate that surface-modifiedartificial hair is highly biosafe and shows excellent tissue adhesion.

Original languageEnglish
Pages (from-to)635-650
Number of pages16
JournalJournal of Adhesion Science and Technology
Volume14
Issue number5
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Keywords

  • Artificial hair
  • Collagen immobilization
  • Interpolymer complex
  • Surface graft polymerization
  • Tissue adhesion

ASJC Scopus subject areas

  • Chemistry(all)
  • Mechanics of Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Materials Chemistry

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