Collagen gel droplet-embedded culture drug sensitivity test (CD-DST) predicts the effect of adjuvant chemotherapy on pancreatic cancer

Kyohei Ariake, Fuyuhiko Motoi, Masamichi Mizuma, Hideo Ohtsuka, Hiroki Hayashi, Kei Nakagawa, Tatsuo Hata, Katsutaka Mitachi, Takeshi Naito, Takashi Kamei, Michiaki Unno

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Purpose: We evaluated the clinical effectiveness of collagen gel droplet-embedded culture drug sensitivity tests (CD-DSTs) in predicting the efficacy of adjuvant chemo-therapeutic treatments for pancreatic cancer (PC). Methods: The clinicopathological characteristics and prognoses of 22 PC patients who underwent CD-DST after pancreatectomy at Tohoku University between 2012 and 2016 were analyzed retrospectively. Eligibility criteria were resectable or borderline resectable PC, successful evaluation for 5-fluorouracil sensitivity by CD-DST, treatment with S-1 adjuvant chemotherapy, and no preoperative chemotherapy. Results: The rate of successful evaluation by CD-DST was 52.3% in PC. The optimal T/C ratio, defined as the ratio of the number of cancer cells in the treatment group (T) to that in the control group (C), for 5-fluorouracil was 85% using receiver operating characteristic curve analysis. The sensitive group (T/C ratio < 85%; n = 11) had a better recurrence-free survival rate than the resistant group (T/C ratio ≥ 85%; n = 11; P = 0.029). A Cox proportional hazards regression model demonstrated that sensitivity to 5-fluorouracil was an independent predictor of recurrence on multivariate analysis (hazard ratio 3.28; 95.0% CI 1.20–9.84; P = 0.020). Conclusions: CD-DSTs helped to predict PC recurrence after S-1 adjuvant chemotherapy.

Original languageEnglish
Pages (from-to)1035-1043
Number of pages9
JournalSurgery today
Volume49
Issue number12
DOIs
Publication statusPublished - 2019 Dec 1

Keywords

  • CD-DST
  • Chemosensitivity test
  • Pancreatic cancer

ASJC Scopus subject areas

  • Surgery

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