Cofilin promotes stimulus-induced lamellipodium formation by generating an abundant supply of actin monomers

Tai Kiuchi, Kazumasa Ohashi, Souichi Kurita, Kensaku Mizuno

Research output: Contribution to journalArticlepeer-review

133 Citations (Scopus)

Abstract

Cofilin stimulates actin filament disassembly and accelerates actin filament turnover. Cofilin is also involved in stimulus-induced actin filament assembly during lamellipodium formation. However, it is not clear whether this occurs by replenishing the actin monomer pool, through filament disassembly, or by creating free barbed ends, through its severing activity. Using photo-activatable Dronpa-actin, we show that cofilin is involved in producing more than half of all cytoplasmic actin monomers and that the rate of actin monomer incorporation into the tip of the lamellipodium is dependent on the size of this actin monomer pool. Finally, in cofilin-depleted cells, stimulus-induced actin monomer incorporation at the cell periphery is attenuated, but the incorporation of micro-injected actin monomers is not. We propose that cofilin contributes to stimulus-induced actin filament assembly and lamellipodium extension by supplying an abundant pool of cytoplasmic actin monomers.

Original languageEnglish
Pages (from-to)465-476
Number of pages12
JournalJournal of Cell Biology
Volume177
Issue number3
DOIs
Publication statusPublished - 2007 May 7

ASJC Scopus subject areas

  • Cell Biology

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