Cofactor-enabled functional expression of fruit fly, honeybee, and bumblebee nicotinic receptors reveals picomolar neonicotinoid actions

Makoto Ihara, Shogo Furutani, Sho Shigetou, Shota Shimada, Kunihiro Niki, Yuma Komori, Masaki Kamiya, Wataru Koizumi, Leo Magara, Mai Hikida, Akira Noguchi, Daiki Okuhara, Yuto Yoshinari, Shu Kondo, Hiromu Tanimoto, Ryusuke Niwa, David B. Sattelle, Kazuhiko Matsuda

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The difficulty of achieving robust functional expression of insect nicotinic acetylcholine receptors (nAChRs) has hampered our understanding of these important molecular targets of globally deployed neonicotinoid insecticides at a time when concerns have grown regarding the toxicity of this chemotype to insect pollinators. We show that thioredoxin-related transmembrane protein 3 (TMX3) is essential to enable robust expression in Xenopus laevis oocytes of honeybee (Apis mellifera) and bumblebee (Bombus terrestris) as well as fruit fly (Drosophila melanogaster) nAChR heteromers targeted by neonicotinoids and not hitherto robustly expressed. This has enabled the characterization of picomolar target site actions of neonicotinoids, findings important in understanding their toxicity.

Original languageEnglish
Pages (from-to)16283-16291
Number of pages9
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number28
DOIs
Publication statusPublished - 2020 Jul 14

Keywords

  • Bumblebee
  • Fruit fly
  • Honeybee
  • Neonicotinoids
  • Nicotinic acetylcholine receptors

ASJC Scopus subject areas

  • General

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