Abstract
Lactobacillus (L.) gasseri JCM1031 has 2 kinds of 6-phospho-β-galactosidase (P-β-gal) in the cytosol. P-β-gal I was already isolated and characterized for our previous paper [Biosci. Biotech. Biochem., 60, 139–141 (1996)]. Another, P-ß-gal II, was purified to homogeneity through several liquid chromatographic steps for this paper. The molecular mass of the purified enzyme was estimated to be 58kDa by SDS–PAGE. The Km and Vmax for ONPGal-6P were 0.76 mM and 86.0 μmol/min/mg, respectively. Reducing reagents and Fe2+ ion activated the enzyme, but PCMB, Zn2+, and Hg2+ ions, and alkylation reagents strongly inhibited it. Twenty-five residues of the N-terminal amino acid sequence of the enzyme were identified. P-β-gal II was different from P-β-gal I in several characteristics.
| Original language | English |
|---|---|
| Pages (from-to) | 708-710 |
| Number of pages | 3 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 60 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1996 Jan |
Keywords
- 6-phospho-β-galactosidase
- Lactobacillus gasseri
- Purification
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry