Coexistence of two kinds of 6-phospho-β-galactosidase in the cytosol of lactobacillus gasseri JCM1031

Masakatsu Suzuki, Tadao Saito, Takatoshi Itoh

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    Lactobacillus (L.) gasseri JCM1031 has 2 kinds of 6-phospho-β-galactosidase (P-β-gal) in the cytosol. P-β-gal I was already isolated and characterized for our previous paper [Biosci. Biotech. Biochem., 60, 139–141 (1996)]. Another, P-ß-gal II, was purified to homogeneity through several liquid chromatographic steps for this paper. The molecular mass of the purified enzyme was estimated to be 58kDa by SDS–PAGE. The Km and Vmax for ONPGal-6P were 0.76 mM and 86.0 μmol/min/mg, respectively. Reducing reagents and Fe2+ ion activated the enzyme, but PCMB, Zn2+, and Hg2+ ions, and alkylation reagents strongly inhibited it. Twenty-five residues of the N-terminal amino acid sequence of the enzyme were identified. P-β-gal II was different from P-β-gal I in several characteristics.

    Original languageEnglish
    Pages (from-to)708-710
    Number of pages3
    JournalBioscience, Biotechnology and Biochemistry
    Volume60
    Issue number4
    DOIs
    Publication statusPublished - 1996 Jan

    Keywords

    • 6-phospho-β-galactosidase
    • Lactobacillus gasseri
    • Purification

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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