Coating of anionic phospholipid polymer onto silica-based microchannel to minimize nonspecific protein adsorption

Yan Xu, Madoka Takai, Tomohiro Konno, Kazuhiko Ishihara

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

An anionic phospholipid copolymer PMBSSi was synthesized to construct a permanent coating with protein resistant units (2-methacryloyloxyethyl phosphorylcholine, MPC) as well as surface charges onto the silica based (quartz and glass) microchannel through a one-step modification. The coating showed a high property of minimizing the non-specific adsorption of both anionic and cationic proteins to a very low extent of less than 0.05 μg /cm2 on glass substrates. In addition, a significant cathodic EOF ((1.0±0.1) ×10-4 cm2/V·s) with approximately one-half of the EOF of the uncoated microchannel was achieved in coated microchannel at neutral pH. As a conclusion, PMBSSi coating is a simple but highly effective modification for reducing nonspecific protein adsorption, and promising to be applied in electrokinetic microfluidic systems.

Original languageEnglish
Pages (from-to)797-800
Number of pages4
JournalKey Engineering Materials
Volume342-343
DOIs
Publication statusPublished - 2007 Jan 1
Externally publishedYes

Keywords

  • Anionic phospholipid polymer
  • Coating
  • Electroosmotic flow (EOF)
  • Microchannel
  • Nonspecific adsorption

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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