Co-localization of glucokinase with actin filaments

Tomiyasu Murata, Hideki Katagiri, Hisamitsu Ishihara, Yoshikazu Shibasaki, Tomoichiro Asano, Yukiyasu Toyoda, Bilgehan Pekiner, Can Pekiner, Ichitomo Miwa, Yoshitomo Oka

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

A portion of glucokinase appeared to be co-localized with actin filaments in the cytoplasm of cultured rat hepatocytes incubated with 25 mM glucose. When liver- or islet-type glucokinase was transiently expressed in COS-7 cells, the expressed glucokinase was also co-localized with actin filaments in the cytoplasm of these transfected cells. Although co-localization of glucokinase with actin filaments was not clearly demonstrated in the pancreatic β-cell line MIN6, islet glucokinase was found to be present in both the nucleus and the cytoplasm, though predominantly in the nucleus. These findings suggest that subcellular localization of glucokinase, including co-localization with actin filaments, may have an important physiological role in metabolic regulation.

Original languageEnglish
Pages (from-to)109-113
Number of pages5
JournalFEBS Letters
Volume406
Issue number1-2
DOIs
Publication statusPublished - 1997 Apr 7

Keywords

  • Actin filament
  • Islet glucokinase
  • Liver glucokinase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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