Cluster-forming property correlated with hemolytic activity by staphylococcal γ-hemolysin transmembrane pores

Noriko Tomita, Kazuyo Abe, Yoshiyuki Kamio, Makoto Ohta

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Staphylococcal γ-hemolysin (Hlg) is a pore-forming toxin consisting of two separate components, LukF (34 kDa) and Hlg2 (32 kDa). Here we show that Hlg pores aggregate and form clusters on human erythrocyte membranes in association with increasing hemolytic activity. Quantitative analysis using transmission electron microscopy and image processing revealed that the formation of single pores and clusters is related to the release of potassium ions and of hemoglobin from erythrocytes, respectively. This is the first study to suggest a novel and unique property which can facilitate hemolysis by the cluster formation of Hlg pores.

Original languageEnglish
Pages (from-to)3452-3456
Number of pages5
JournalFEBS Letters
Volume585
Issue number21
DOIs
Publication statusPublished - 2011 Nov 4

Keywords

  • Cluster formation
  • Image analysis
  • Pore-forming toxin
  • Staphylococcal γ-hemolysin
  • Transmission electron microscopy
  • Two-component cytolysin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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