Clostridium pabulibutyricum sp. Nov., a butyric-acid-producing organism isolated from high-moisture grass silage

Hisami Kobayashi, Takuya Nakasato, Mitsuo Sakamoto, Yoshihisa Ohtani, Fuminori Terada, Ken Sakai, Moriya Ohkuma, Masanori Tohno

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    A Gram-stain-variable, strictly anaerobic, rod-shaped, catalase-negative and endospore-forming bacterial strain, designated MJC39T, was isolated from grass silage preserved in Hokkaido, Japan. Growth occurred at 20–42 °C, pH 5.0–7.0 and NaCl concentrations up to 2% (w/v). The isolated strain MJC39T produced butyric acid in peptone yeast extract medium with glucose. The DNA G+C content of strain MJC39T was 34.4±0.2 mol%. The major cellular fatty acids (>10 %) were C14: 0, C16: 0 and summed feature 3 (including C16: 1 ω7c/C16: 1 ω6c). No respiratory quinones were detected. The polar lipids of strain MJC39T were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, one unidentified lipid, one unidentified aminolipid, two unidentified glycolipids, one unidentified phospholipid, one unidentified aminoglycolipid and one unidentified phosphoaminoglycolipid. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJC39T was a member of the genus Clostridium and is closely related to Clostridium tyrobutyricum JCM 11008T (95.8% similarity) and Clostridium algifaecis MB9-7T (95.5% similarity). Based on the genotypic, phenotypic and chemotaxonomic characteristics, strain MJC39T represents a novel species of the genus Clostridium, for which the name Clostridium pabulibutyricum sp. nov. is proposed. The type strain is MJC39T (=JCM 31506T=DSM 103944T).

    Original languageEnglish
    Article number002387
    Pages (from-to)4974-4978
    Number of pages5
    JournalInternational journal of systematic and evolutionary microbiology
    Volume67
    Issue number12
    DOIs
    Publication statusPublished - 2017 Dec

    Keywords

    • Clostridium pabulibutyricum sp. nov
    • Silage

    ASJC Scopus subject areas

    • Microbiology
    • Ecology, Evolution, Behavior and Systematics

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