Cloning of the protein D2 gene of Pseudomonas aeruginosa and its functional expression in the imipenem-resistant host

Hiroshi Yoneyama, Taiji Nakac

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Protein D2 forms the water-filled pore across the outer membrane of Pseudomonas aeruginosa and allows the penetration of imipenem. We cloned the protein D2 gene by the antibody screening technique. When the imipenem-resistant mutant lacking protein D2 harbored the plasmid with the cloned D2 gene, the mutant overproduced protein D2 in the outer membrane. These transformants exhibited fully-restored imipenem susceptibility. The results prove unequivocally that protein D2 forms the imipenem-permeable pore in the P. aeruginosa outer membrane.

Original languageEnglish
Pages (from-to)177-179
Number of pages3
JournalFEBS Letters
Volume283
Issue number2
DOIs
Publication statusPublished - 1991 Jun 3
Externally publishedYes

Keywords

  • Imipenem
  • Outer membrane
  • Permeability
  • Porin
  • Protein D2
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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