Cloning of the Microcystis aeruginosa M228 lectin (MAL) gene

Mitsuru Jimbo, Masato Yamaguchi, Koji Muramoto, Hisao Kamiya

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

We have cloned and characterized the gene encoding Microcystis aeruginosa (strain M228) lectin (MAL). The gene contains 1551 nucleotides and an open reading frame for a protein of 517 amino acids with a predicted molecular weight of 55,159 Da. The carboxyterminal region of MAL has three tandemly repeated homologous domains composed of 61 amino acids. These regions show similarity to the corresponding regions of the α-amylase of Clostridium beijerinckii (23% identity). The mal gene lies adjacent to an ORF that dislay homology to cytochrome P-450 and polyketide synthase. Southern hybridization showed that the genomic DNA of the strain M228 contained, in addition to MAL gene (mal), at least two other mal like gene. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)499-504
Number of pages6
JournalBiochemical and biophysical research communications
Volume273
Issue number2
DOIs
Publication statusPublished - 2000 Jul 5
Externally publishedYes

Keywords

  • Cyanobacterium
  • Gene
  • Lectin
  • Microcystis aeruginosa
  • Polyketide synthase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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