TY - JOUR
T1 - Cloning of the genome of equine herpesvirus 4 strain TH20p as an infectious bacterial artificial chromosome
AU - Azab, Walid
AU - Kato, Kentaro
AU - Arii, Jun
AU - Tsujimura, Koji
AU - Yamane, Daisuke
AU - Tohya, Yukinobu
AU - Matsumura, Tomio
AU - Akashi, Hiroomi
N1 - Funding Information:
The authors are grateful to Dr. A. Vanderplasschen, University of Liege, and Dr. N. Osterrieder, Cornell University, for useful discussions and technical advice. This work was supported in part by grants from the Egyptian Ministry of Higher Education and a Grant-in-Aid for Scientific Research in Priority Areas from the Ministry of Education, Culture, Science, Sports and Technology (MEXT) of Japan.
PY - 2009/5
Y1 - 2009/5
N2 - Equine herpesvirus 4 (EHV-4) is a major cause of respiratory tract disease in horses worldwide. The generation of recombinant viruses, which would lead to understanding of viral gene functions, has been hindered by the absence of suitable cell lines and small-animal models of the infection. In the present study, the genome of EHV-4 strain TH20p was cloned as a stable and infectious BAC without any deletions of the viral genes. Mini F plasmid sequences flanked by loxP sites were inserted into the intergenic region between genes 58 and 59. Coinfection of the recombinant virus with a recombinant adenovirus expressing Cre recombinase resulted in the excision of the BAC sequences. Importantly, the resulting recombinant EHV-4 replicated comparably to the wild-type virus in fetal horse kidney cells. The recombinant EHV-4 will facilitate EHV-4 research and provide the opportunity to exploit the power of BAC technology for production of recombinant viral vaccines.
AB - Equine herpesvirus 4 (EHV-4) is a major cause of respiratory tract disease in horses worldwide. The generation of recombinant viruses, which would lead to understanding of viral gene functions, has been hindered by the absence of suitable cell lines and small-animal models of the infection. In the present study, the genome of EHV-4 strain TH20p was cloned as a stable and infectious BAC without any deletions of the viral genes. Mini F plasmid sequences flanked by loxP sites were inserted into the intergenic region between genes 58 and 59. Coinfection of the recombinant virus with a recombinant adenovirus expressing Cre recombinase resulted in the excision of the BAC sequences. Importantly, the resulting recombinant EHV-4 replicated comparably to the wild-type virus in fetal horse kidney cells. The recombinant EHV-4 will facilitate EHV-4 research and provide the opportunity to exploit the power of BAC technology for production of recombinant viral vaccines.
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U2 - 10.1007/s00705-009-0382-0
DO - 10.1007/s00705-009-0382-0
M3 - Article
C2 - 19387789
AN - SCOPUS:65549138008
VL - 154
SP - 833
EP - 842
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 5
ER -