Cloning of cDNA encoding the rice 22 kDa protein of Photosystem II (PSII-S) and analysis of light-induced expression of the gene

Toshisuke Iwasaki, Yoko Saito, Erimi Harada, Makoto Kasai, Kazuhiro Shoji, Mitsue Miyao, Naoki Yamamoto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cloning of rice cDNA encoding the chlorophyll-binding 22 kDa protein of Photosystem II (PSII-S) and the light-induced expression of the gene are reported. One of the light-responsive cDNA clones, isolated by screening with a light-specific subtracted cDNA probe, was shown to encode PSII-S of rice. Genomic Southern analysis suggested that the PSII-S gene, psbS, is a single-copy gene in rice. A brief exposure to red light induced a severalfold increase in the steady state level of PSII-S transcripts in etiolated seedlings. The red light effect was reversed by far-red light, suggesting involvement of phytochrome in the PSII-S gene regulation. Prolonged exposure (3 h) to blue light, however, revealed a much stronger effect than red light on the accumulation of PSII-S transcripts in the etiolated seedlings. In dark-adapted green plants, prolonged exposure to blue light induced re-accumulation of transcripts encoding PSII-S, whereas red light had little effect.

Original languageEnglish
Pages (from-to)223-229
Number of pages7
JournalGene
Volume185
Issue number2
DOIs
Publication statusPublished - 1997 Feb 7
Externally publishedYes

Keywords

  • Cp26
  • ELIP
  • Oryza sativa
  • psbS

ASJC Scopus subject areas

  • Genetics

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