Cloning of a cDNA that encodes farnesyl diphosphate synthase and the blue-light-induced expression of the corresponding gene in the leaves of rice plants

Kazutsuka Sanmiya, Toshisuke Iwasaki, Makoto Matsuoka, Mitsue Miyao, Naoki Yamamoto

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A cDNA encoding farnesyl diphosphate synthase (FPPS), a key enzyme in isoprenoid biosynthesis, was isolated from a cDNA library constructed from mRNA that had been prepared from etiolated rice (Oriza sativa L. variety Nipponbare) seedlings after three hours of illumination by a subtraction method. The putative polypeptide deduced from the 1289 bp nucleotide sequence consisted of 353 amino acids and had a molecular mass of 40676 Da. The predicted amino acid sequence exhibited high homology to those of FPPS from Arabidopsis (73% to type 1, 72% to type 2) and white lupin (74%). Southern blot analysis showed that the rice genome might contain only one gene for FPPS. The highest level of expression of the gene was demonstrated in leaves by RNA blot analysis. Moreover, light, in particular blue light, effectively enhanced expression of the gene.

Original languageEnglish
Pages (from-to)240-246
Number of pages7
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1350
Issue number3
DOIs
Publication statusPublished - 1997 Mar 28
Externally publishedYes

Keywords

  • Oriza sativa
  • cDNA
  • dark adaptation
  • etiolated seedling
  • farnesyl diphosphate synthase
  • light-responsive gene

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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