Cloning of a cDNA encoding an importin-α and down-regulation of the gene by light in rice leaves

Kazuhiro Shoji, Toshisuke Iwasaki, Rikyu Matsuki, Mitsue Miyao, Naoki Yamamoto

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The import of nuclear proteins into nuclei begins with recognition of nuclear localization signal-harboring proteins and binding to a nuclear pore targeting complex. A cDNA for an importin-α protein, a subunit of the complex, was isolated from rice plants. The amino acid sequence deduced from the nucleotide sequence of the cDNA exhibited a high homology to those of importin-α proteins from many organisms such as Arabidopsis thaliana, Saccharomyces cerevisiae, human, mouse, Xenopus laevis and Drosophila melanogaster. Down-regulation of the transcription by light was shown in the leaves of light- and dark-grown seedlings by RNA blot analysis. The down-regulation was specific to leaves, whereas no light effect was observed in root tissues or calli, in which higher levels of the transcript were detected.

Original languageEnglish
Pages (from-to)279-286
Number of pages8
JournalGene
Volume212
Issue number2
DOIs
Publication statusPublished - 1998 Jun 8
Externally publishedYes

Keywords

  • Light regulation of transcription
  • NLS
  • Nuclear protein import
  • Orysa sativa

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Cloning of a cDNA encoding an importin-α and down-regulation of the gene by light in rice leaves'. Together they form a unique fingerprint.

Cite this