Cloning of a calcitonin gene-related peptide receptor and a novel calcitonin receptor-like receptor from the gill of flounder, Paralichthys olivaceus

Nobuo Suzuki, Tohru Suzuki, Tadahide Kurokawa

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

For the first time in non-mammalian vertebrates, cDNA encoding CGRPR was isolated from the gill cDNA library of flounder. The nucleotide sequence consists of a 237 bp 5'-UTR, a 1398 bp coding sequence for a 465-amino-acid protein, and a 981 bp 3'-UTR. The predicted amino-acid sequence has a high degree of identity to hCGRPR (72.3%) and rCGRPR (71.6%) and, to a lesser degree, to hCTR (55.6%) and rCTR (59.3%). In addition, a different type of receptor cDNA was also obtained from the gill cDNA. The nucleotide sequence contains an open-reading frame of 1380 bp to produce a 459-amino-acid protein. The open-reading frame of this receptor shows the same degree of identity to mammalian CTR (60.2% to hCTR; 62.3% to rCTR) and CGRPR (64.6% to hCGRPR; 64.4% to rCGRPR). However, the predicted amino-acid sequence was more homologous to hCGRPR (60.2%) and rCGRPR (61.3%) than to hCTR (48.8%) and rCTR (49.5%). The identity of this receptor to fCGRPR is 66.6% at the nucleotide level and 64.2% at the amino-acid level, indicating that the receptor is not likely to be an isoform of CGRPR. The receptor, but not fCGRPR, is expressed in bones, suggesting the possibility that this receptor corresponds to the flounder CTR. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalGene
Volume244
Issue number1-2
DOIs
Publication statusPublished - 2000 Feb 22
Externally publishedYes

Keywords

  • Binding sites
  • RT-PCR
  • Teleost
  • Tissue expression

ASJC Scopus subject areas

  • Genetics

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