Cloning of a calcitonin gene-related peptide from genomic DNA and its mRNA expression in flounder, Paralichthys olivaceus

Nobuo Suzuki, Tohru Suzuki, Tadahide Kurokawa

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

A part of genomic DNA including the calcitonin gene-related peptide (CGRP) gene was cloned from flounder by the genome-walking method. The intron/exon boundary was predicted to occur exactly at the same position as in salmon. The 37-amino acid molecule coded by the region from the intron/exon boundary to the stop codon was preceded by a typical Lys-Arg cleavage signal and included a cleavage/amidation site common to the CGRP of other vertebrates. The predicted amino acid sequence of flounder CGRP had 78%, 78%, 78%, 81%, and 73-78% identity to that of salmon, cod, frog, chicken, and mammalian CGRPs, respectively. Among vertebrates, CGRP is more conserved than calcitonin (CT) because the identity of flounder CT to mammalian CTs is 31-50%. Expression analysis indicated that this hormone is synthesized in the brain, heart, intestine, testis, and ovary. Since we have previously shown that the CGRP receptor is expressed in these tissues, it is suggested that CGRP secreted from each tissue functions in a paracrine or autocrine manner.

Original languageEnglish
Pages (from-to)1435-1438
Number of pages4
JournalPeptides
Volume22
Issue number9
DOIs
Publication statusPublished - 2001 Aug 31
Externally publishedYes

Keywords

  • Calcitonin
  • Calcitonin gene-related peptide
  • Flounder
  • Genome walking method
  • Polymerase chain reaction (PCR)
  • Tissue expression

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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