Cloning of γ-hexachlorocyclohexane dehydrochlorinase gene with its flanking regions from soil by activity-based screening techniques

Michihiro Ito, Akira Ono, Yoshiyuki Otsubo, Masataka Tsuda, Yuji Nagata

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Activity-based screening techniques were applied to clone a gene encoding γ-hexachlorocyclohexane (γ-HCH) dehydrochlorinase with its flanking regions from a cosmid-based library of DNA that was extracted from a γ-HCH-added suspension of HCH-contaminated soil. A total of 11 cosmid clones showing the γ-HCH dehydrochlorinase activity were obtained through the screenings, indicating the validity of this approach. All the clones had a linA gene identical to known one, but its flanking regions showed some structural variations with known ones, suggesting high likelihood of genetic divergence in the linA-flanking regions.

    Original languageEnglish
    Pages (from-to)16-19
    Number of pages4
    JournalEuropean Journal of Soil Biology
    Volume52
    DOIs
    Publication statusPublished - 2012 Sep 1

    Keywords

    • Biodegradation
    • Dehalogenase
    • Dehydrochlorinase
    • Xenobiotics
    • γ-Hexachlorocyclohexane

    ASJC Scopus subject areas

    • Microbiology
    • Soil Science
    • Insect Science

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