TY - JOUR
T1 - Cloning in Escherichia coli of the structural gene of prorennin, the precursor of calf milk-clotting enzyme rennin
AU - Nishimori, Katsuhiko
AU - Kawaguchi, Yoshiyuki
AU - Hidaka, Makoto
AU - Uozumi, Takeshi
AU - Beppu, Teruhiko
PY - 1981/1/1
Y1 - 1981/1/1
N2 - Double-stranded cDNA was prepared from prorennin-specific mRNA by sequential actions of reverse transcriptase, DNA polymerase and SI nuclease, and inserted into the SalI site of pBR322 by the poly(dG)-(dC) annealing method. Transformation of Escherichia coli C600 r- m- by the hybrid plasmid yielded transformants containing prorennin cDNA. The presence of the cDNA sequence in these clones was confirmed by both colony hybridization and hybrid-arrested translation of the mRNA in vitro. The largest size of the cloned cDNA was 1,020 bp.
AB - Double-stranded cDNA was prepared from prorennin-specific mRNA by sequential actions of reverse transcriptase, DNA polymerase and SI nuclease, and inserted into the SalI site of pBR322 by the poly(dG)-(dC) annealing method. Transformation of Escherichia coli C600 r- m- by the hybrid plasmid yielded transformants containing prorennin cDNA. The presence of the cDNA sequence in these clones was confirmed by both colony hybridization and hybrid-arrested translation of the mRNA in vitro. The largest size of the cloned cDNA was 1,020 bp.
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U2 - 10.1093/oxfordjournals.jbchem.a133550
DO - 10.1093/oxfordjournals.jbchem.a133550
M3 - Article
C2 - 6171564
AN - SCOPUS:0019456272
VL - 90
SP - 901
EP - 904
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 3
ER -