Cloning in Escherichia coli of the structural gene of prorennin, the precursor of calf milk-clotting enzyme rennin

Katsuhiko Nishimori, Yoshiyuki Kawaguchi, Makoto Hidaka, Takeshi Uozumi, Teruhiko Beppu

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Double-stranded cDNA was prepared from prorennin-specific mRNA by sequential actions of reverse transcriptase, DNA polymerase and SI nuclease, and inserted into the SalI site of pBR322 by the poly(dG)-(dC) annealing method. Transformation of Escherichia coli C600 r- m- by the hybrid plasmid yielded transformants containing prorennin cDNA. The presence of the cDNA sequence in these clones was confirmed by both colony hybridization and hybrid-arrested translation of the mRNA in vitro. The largest size of the cloned cDNA was 1,020 bp.

Original languageEnglish
Pages (from-to)901-904
Number of pages4
JournalJournal of biochemistry
Volume90
Issue number3
DOIs
Publication statusPublished - 1981 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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