Cloning, chromosomal localization, and functional expression of the α1 subunit of the L-type voltage-dependent calcium channel from normal human heart

D. Schultz, G. Mikala, A. Yatani, D. B. Engle, D. E. Iles, B. Segers, R. J. Sinke, D. O. Weghuis, U. Klockner, M. Wakamori, J. J. Wang, D. Melvin, G. Varadi, A. Schwartz

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Abstract

A unique structural variant of the cardiac L-type voltage-dependent calcium channel α1 subunit cDNA was isolated from libraries derived from normal human heart mRNA. The deduced amino acid sequence shows significant homology to other calcium channel α1 subunits. However, differences from the rabbit heart α1 include a shortened N-terminus, a unique C-terminal insertion, and both forms of an alternatively spliced motif IV S3 region. The shortened N-terminus provides optimal access to consensus sequences thought to facilitate translation. Northern blot analysis revealed a single hybridizing mRNA species of 9.4 kb. The gene for the human heart α1 subunit was localized specifically to the distal region of chromosome 12p13. The cloned α1 subunit was expressed in Xenopus oocytes and single-channel analyses revealed native-like pharmacology and channel properties.

Original languageEnglish
Pages (from-to)6228-6232
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number13
DOIs
Publication statusPublished - 1993 Jan 1
Externally publishedYes

ASJC Scopus subject areas

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    Schultz, D., Mikala, G., Yatani, A., Engle, D. B., Iles, D. E., Segers, B., Sinke, R. J., Weghuis, D. O., Klockner, U., Wakamori, M., Wang, J. J., Melvin, D., Varadi, G., & Schwartz, A. (1993). Cloning, chromosomal localization, and functional expression of the α1 subunit of the L-type voltage-dependent calcium channel from normal human heart. Proceedings of the National Academy of Sciences of the United States of America, 90(13), 6228-6232. https://doi.org/10.1073/pnas.90.13.6228