Cloning and transcript analysis of multiple genes encoding the glycoside hydrolase family 6 enzyme from Coprinopsis cinerea

Makoto Yoshida, Kan Sato, Satoshi Kaneko, Kiyoharu Fukuda

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We searched the genome database of the basidiomycete Coprinopsis cinerea (Coprinus cinereus) and found five genes encoding the glycoside hydrolase family 6 (GH6) enzyme, CcCel6A, CcCel6B, CcCel6C, CcCel6D, and CcCel6E, designated in order of increasing locus number (CC1G-01107.1, CC1G-04166.1, CC1G-08276.1, CC1G-08277.1, and CC1G-10605.1). The amino acid sequence of CcCel6A suggests a two-domain structure consisting of an N-terminal family 1 carbohydrate-binding module (CBM1) and a GH6 catalytic domain, while the other genes lack CBM1. The transcripts of CcCel6A were observed at the active growth stage in cellulose culture, whereas they were absent from glucose culture. Cellobiose strongly induced transcription of CcCel6A. On the other hand, transcripts of CcCel6B, -D, and -E were detected in both glucose and cellulose cultures, and transcription of them was induced weakly by cellobiose. The transcript level of CcCel6C was not influenced by glucose or cellobiose.

Original languageEnglish
Pages (from-to)67-73
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number1
DOIs
Publication statusPublished - 2009 Feb 2
Externally publishedYes

Keywords

  • Basidiomycete
  • Cellulose degradation
  • Coprinopsis cinerea
  • Glycoside hydrolase family 6

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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