Cloning and sequencing of the gene encoding the plasma membrane H+-ATPase from an acidophilic red alga, Cyanidium caldarium

Hisataka Ohta, Hitoshi Shirakawa, Kohji Uchida, Michiteru Yoshida, Yuhsi Matuo, Isao Enami

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

A cDNA containing an open reading frame encoding the putative plasma membrane H+-ATPase in an acidophilic red alga, Cyanidium caldarium, was cloned and sequenced by means of PCR and Southern hybridization based on homologous sequences of P-type ATPases found in other organisms. The cloned cDNA is 3300 bp in length, containing a 2865 bp open reading frame encoding a polypeptide of 955 amino acids which has a predicted molecular mass of 105 371. The deduced amino acid sequence was found to be more homologous to those of P-type H+-ATPases from higher plants than that from the green alga Dunaliella bioculata.

Original languageEnglish
Pages (from-to)9-13
Number of pages5
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1319
Issue number1
DOIs
Publication statusPublished - 1997 Mar 28

Keywords

  • Acidophilic red alga
  • Cyanidium caldarium
  • Intracellular pH regulation
  • P-type H-ATPase
  • cDNA sequence

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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