Cloning and sequence analysis of czc genes in alcaligenes sp. Strain CT14

Takashi Kunito; Tomonobu Kusano; Hiroshi Oyaizu; Keishi Senoo; Shinjiro Kanazawa; Satoshi Matsumoto

doi:10.1271/bbb.60.699

Cloning and sequence analysis of czc genes in alcaligenes sp. Strain CT14

Takashi Kunito, Tomonobu Kusano, Hiroshi Oyaizu, Keishi Senoo, Shinjiro Kanazawa, Satoshi Matsumoto

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

We have isolated 14 cadmium (Cd)-resistant, soil-borne bacteria. Among those, strain CT14, which was identified as an Alcatigenes sp., has a czc (cadmium, zinc, and cobalt divalent cation resistant determinant) system. Here we report the nucleotide sequence of 4 genes (czcCBAD) of the system. CzcCBA showed over 98% identity with those of A. eutrophus CH34, however, CzcD, the distal gene product, was 117 amino acids longer than that (199 amino acids) of A. eutrophus CH34, and had considerable similarity to the members of the CDF (cation diffusion facilitator) family proteins all over the region.

Original language	English
Pages (from-to)	699-704
Number of pages	6
Journal	Bioscience, Biotechnology and Biochemistry
Volume	60
Issue number	4
DOIs	https://doi.org/10.1271/bbb.60.699
Publication status	Published - 1996 Jan

Keywords

Alcaligenes eutrophus
Czcsystem
Heavy metal
Resistance

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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abstract = "We have isolated 14 cadmium (Cd)-resistant, soil-borne bacteria. Among those, strain CT14, which was identified as an Alcatigenes sp., has a czc (cadmium, zinc, and cobalt divalent cation resistant determinant) system. Here we report the nucleotide sequence of 4 genes (czcCBAD) of the system. CzcCBA showed over 98% identity with those of A. eutrophus CH34, however, CzcD, the distal gene product, was 117 amino acids longer than that (199 amino acids) of A. eutrophus CH34, and had considerable similarity to the members of the CDF (cation diffusion facilitator) family proteins all over the region.",

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AU - Kunito, Takashi

AU - Kusano, Tomonobu

AU - Oyaizu, Hiroshi

AU - Senoo, Keishi

AU - Kanazawa, Shinjiro

AU - Matsumoto, Satoshi

PY - 1996/1

Y1 - 1996/1

N2 - We have isolated 14 cadmium (Cd)-resistant, soil-borne bacteria. Among those, strain CT14, which was identified as an Alcatigenes sp., has a czc (cadmium, zinc, and cobalt divalent cation resistant determinant) system. Here we report the nucleotide sequence of 4 genes (czcCBAD) of the system. CzcCBA showed over 98% identity with those of A. eutrophus CH34, however, CzcD, the distal gene product, was 117 amino acids longer than that (199 amino acids) of A. eutrophus CH34, and had considerable similarity to the members of the CDF (cation diffusion facilitator) family proteins all over the region.

AB - We have isolated 14 cadmium (Cd)-resistant, soil-borne bacteria. Among those, strain CT14, which was identified as an Alcatigenes sp., has a czc (cadmium, zinc, and cobalt divalent cation resistant determinant) system. Here we report the nucleotide sequence of 4 genes (czcCBAD) of the system. CzcCBA showed over 98% identity with those of A. eutrophus CH34, however, CzcD, the distal gene product, was 117 amino acids longer than that (199 amino acids) of A. eutrophus CH34, and had considerable similarity to the members of the CDF (cation diffusion facilitator) family proteins all over the region.

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KW - Heavy metal

KW - Resistance

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Cloning and sequence analysis of czc genes in alcaligenes sp. Strain CT14

Abstract

Keywords

ASJC Scopus subject areas

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