Cloning and sequence analysis of cDNA for rat corticotropin-releasing factor precursor

Hisato Jingami; Noboru Mizuno; Hideo Takahashi; Shigeki Shibahara; Yasuji Furutani; Hiroo Imura; Shosaku Numa

doi:10.1016/0014-5793(85)80994-7

Cloning and sequence analysis of cDNA for rat corticotropin-releasing factor precursor

Hisato Jingami, Noboru Mizuno, Hideo Takahashi, Shigeki Shibahara, Yasuji Furutani, Hiroo Imura, Shosaku Numa

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

183 Citations (Scopus)

Abstract

DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including a putative signal peptide. The CRF and putative signal peptide regions are more highly conserved among rat, human and ovine prepro-CRF than is the cryptic portion.

Original language	English
Pages (from-to)	63-66
Number of pages	4
Journal	FEBS Letters
Volume	191
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(85)80994-7
Publication status	Published - 1985 Oct 21

Keywords

Corticotropin-releasing factor
Hormone precursor
Nucleotide sequence
cDNA cloning

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(85)80994-7

Cite this

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title = "Cloning and sequence analysis of cDNA for rat corticotropin-releasing factor precursor",

abstract = "DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including a putative signal peptide. The CRF and putative signal peptide regions are more highly conserved among rat, human and ovine prepro-CRF than is the cryptic portion.",

keywords = "Corticotropin-releasing factor, Hormone precursor, Nucleotide sequence, cDNA cloning",

author = "Hisato Jingami and Noboru Mizuno and Hideo Takahashi and Shigeki Shibahara and Yasuji Furutani and Hiroo Imura and Shosaku Numa",

note = "Funding Information: We thank Drs Paul Berg and Hiroto Okayama for providing their high-efficiency cloning system, Dr Takashi Miyata for computer analysis and Dr Shigetada Nakanishi for helpful advice. This investigation was supported in part by research grants from the Ministry of Education, Science and Culture of Japan, the Mitsubishi Foundation and the Japanese Foundation of Metabolism and Diseases.",

year = "1985",

month = oct,

day = "21",

doi = "10.1016/0014-5793(85)80994-7",

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T1 - Cloning and sequence analysis of cDNA for rat corticotropin-releasing factor precursor

AU - Jingami, Hisato

AU - Mizuno, Noboru

AU - Takahashi, Hideo

AU - Shibahara, Shigeki

AU - Furutani, Yasuji

AU - Imura, Hiroo

AU - Numa, Shosaku

N1 - Funding Information: We thank Drs Paul Berg and Hiroto Okayama for providing their high-efficiency cloning system, Dr Takashi Miyata for computer analysis and Dr Shigetada Nakanishi for helpful advice. This investigation was supported in part by research grants from the Ministry of Education, Science and Culture of Japan, the Mitsubishi Foundation and the Japanese Foundation of Metabolism and Diseases.

PY - 1985/10/21

Y1 - 1985/10/21

N2 - DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including a putative signal peptide. The CRF and putative signal peptide regions are more highly conserved among rat, human and ovine prepro-CRF than is the cryptic portion.

AB - DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including a putative signal peptide. The CRF and putative signal peptide regions are more highly conserved among rat, human and ovine prepro-CRF than is the cryptic portion.

KW - Corticotropin-releasing factor

KW - Hormone precursor

KW - Nucleotide sequence

KW - cDNA cloning

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DO - 10.1016/0014-5793(85)80994-7

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JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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ER -

Cloning and sequence analysis of cDNA for rat corticotropin-releasing factor precursor

Abstract

Keywords

ASJC Scopus subject areas

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