Cloning and sequence analysis of cDNA for rat corticotropin-releasing factor precursor

Hisato Jingami, Noboru Mizuno, Hideo Takahashi, Shigeki Shibahara, Yasuji Furutani, Hiroo Imura, Shosaku Numa

Research output: Contribution to journalArticlepeer-review

183 Citations (Scopus)

Abstract

DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including a putative signal peptide. The CRF and putative signal peptide regions are more highly conserved among rat, human and ovine prepro-CRF than is the cryptic portion.

Original languageEnglish
Pages (from-to)63-66
Number of pages4
JournalFEBS Letters
Volume191
Issue number1
DOIs
Publication statusPublished - 1985 Oct 21

Keywords

  • Corticotropin-releasing factor
  • Hormone precursor
  • Nucleotide sequence
  • cDNA cloning

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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