Cloning and nucleotide sequence of the genomic ribonuclease T2 gene (rntB) from Aspergillus oryzae

Kenji Ozeki, Katsuhiko Kitamoto, Katsuya Gomi, Chieko Kumagai, Gakuzo Tamura, Shodo Hara

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Using synthetic oligonucleotide probes, we have cloned a genomic DNA sequence encoding a ribonuclease (RNase) T2 gene (rntB) from Aspergillus oryzae on a 4.8 kb HindIII fragment. DNA sequence analysis of the RNase T2 revealed the following: (1) The gene is arranged as five exons and four introns; (2) The deduced amino acid sequence contains 239 amino acid residues of the mature enzyme. In addition, there exist 17 amino acid residues thought to be a signal peptide sequence at the N-terminus and 20 amino acid residues at the C-terminus; (3) The nucleotide sequence of the rntB gene is homologous to those of the RNase Rh gene from Rhizopus niveus and the S2 stylar glycoprotein gene of Nicotiana alata with degree of about 51% and 47%, respectively; (4) A. oryzae and A. nidulans transformed with the cloned rntB gene had much higher ribonuclease T2 activity than wild-type strains.

Original languageEnglish
Pages (from-to)367-373
Number of pages7
JournalCurrent Genetics
Volume19
Issue number5
DOIs
Publication statusPublished - 1991 May 1
Externally publishedYes

Keywords

  • Aspergillus oryzae
  • Nucleotide sequence
  • Ribonuclease T
  • Transformation

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Cloning and nucleotide sequence of the genomic ribonuclease T<sub>2</sub> gene (rntB) from Aspergillus oryzae'. Together they form a unique fingerprint.

  • Cite this