Cloning and genomic characterization of sytdep, a new synaptotagmin XIV-related gene

M. Javier Herrero-Turrión, Mitsunori Fukuda, Faustino Mollinedo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have identified a new human gene coined sytdep (synaptotagmin XIV-derived protein) in human neutrophils. Sytdep encodes a 188-amino acid sequence with a 21.435 kDa deduced molecular mass, showing 75% identity to human synaptotagmin (syt) XIV. Human neutrophils express sytdep, but not syt XIV. Sytdep was upregulated during HL-60 neutrophil differentiation. Sytdep gene is located in human chromosome 4 and contains a unique exon, whereas syt XIV gene, located in chromosome 1, comprises 10 exons with 9 introns. Mouse genome did not contain sytdep. The N-terminal region of sytdep shows no homology with any known protein and, unlike synaptotagmin XIV isoforms, sytdep shows a unique C-terminal C2B domain. Polyclonal antibodies against the C2B domain of syt XIV recognized sytdep as a 27-kDa protein in human neutrophils. Genomic analyses suggest that human sytdep could derive from a retrotranslocation of a syt XIV transcript into chromosome 4.

Original languageEnglish
Pages (from-to)386-394
Number of pages9
JournalBiochemical and biophysical research communications
Volume340
Issue number2
DOIs
Publication statusPublished - 2006 Feb 10
Externally publishedYes

Keywords

  • Alternative splicing
  • C2 domain
  • Cloning
  • Gene expression
  • Genomic characterization
  • Human neutrophils
  • Synaptotagmin XIV-derived gene

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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