Cloning and expression pattern of a novel microspore-specific gene encoding hypersensitive-induced response protein (LjHIR1) from the model legume, Lotus japonicus

Hirokazu Hakozaki, Makoto Endo, Hiromi Masuko, Jong In Park, Hitoshi Ito, Masanori Uchida, Motoshi Kamada, Hideyuki Takahashi, Atsushi Higashitani, Masao Watanabe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In order to understand the microspore and pollen development, recently, we have isolated a number of anther-specific genes in the model legume, Lotus japonicus. From these anther-specific genes, we identified one novel microspore-specific gene, LjImfb-c82. In order to determine the molecular characterization of LjImfb-c82, full-length cDNA clone was first isolated and sequenced. It encoded a protein of 286 amino acids (LjHIR1), which had sequence similarity to Hypersensitive-Induced Response like protein. LjHIR1 was specifically expressed in microspore on the in situ hybridization experiment. From the sequence similarity to prohibitin-domain protein, the LjHIR1 might be related to ion channel regulation in microspore development.

Original languageEnglish
Pages (from-to)307-310
Number of pages4
JournalGenes and Genetic Systems
Volume79
Issue number5
DOIs
Publication statusPublished - 2004 Oct 1

Keywords

  • Hypersensitive-induced response (HIR) genes
  • Lotus japonicus
  • Microspore-specific gene
  • Prohibitin-domain proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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