Cloning and expression of type XII collagen isoforms during bovine adipogenesis

Koji Tahara, Hisashi Aso, Tadashi Yamasaki, Michael T. Rose, Akiko Takasuga, Yoshikazu Sugimoto, Takahiro Yamaguchi, Shoichi Takano

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

In order to isolate candidate genes involved in bovine adipocyte differentiation, we have constructed a subtraction library from a clonal bovine intra-muscular pre-adipocyte (BIP) cell line using the suppression subtractive hybridization method. We have isolated a set of subtracted cDNA fragments whose respective mRNA levels are up-regulated during the adipogenic differentiation of BIP cells, and cloned cDNAs from a differentiated BIP-λ ZAP II cDNA library. Two cDNA clones were highly homologous to the sequence- of mouse and human type XII collagen a-1, determined by a BLAST homology search. As type XII collagen has been reported to have four types of splicing isoform, two clones were determined to be XII-1 and XII-2 splicing isoforms, respectively, because of a difference in the C-terminal NC1 domain. From the expression analysis of type XII collagen, the XIIA-2 isoform was mainly expressed in differentiated BIP cells and adipose tissues. Although the function of type XII collagen has not been established as yet, these results suggest that type XII collagen may be associated with adipocyte differentiation and adipose formation in cattle and is a potentially useful marker for adipogenesis.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalDifferentiation
Volume72
Issue number4
DOIs
Publication statusPublished - 2004 Apr

Keywords

  • Adipogenesis
  • BIP cell
  • Subtraction
  • Type XII collagen

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

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