Cloning and expression of the exo-β-d-1,3-glucanase gene (exgs) from aspergillu saitoi

Ken Oda; Shin Kasahara; Youhei Yamagata; Keietsu Abe; Tasuku Nakajima

doi:10.1271/bbb.66.1587

Cloning and expression of the exo-β-d-1,3-glucanase gene (exgs) from aspergillu saitoi

Ken Oda, Shin Kasahara, Youhei Yamagata, Keietsu Abe, Tasuku Nakajima

AGR - Bioscience and Biotechnology for Future Bioindustries

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A gene of exo-1,3-β-D-glucanase (exgS) was cloned from a koji mold, Aspergillus saitoi, genomic DNA using PCR. The exgS has an ORF comprising 2832 bp, which contains one intron of 45 bp, and encodes 945 amino acids. The deduced amino acid sequences showed that the ExgS has a non-homologous linker region consisting of 180 amino acids, which encompassed highly conserved regions observed in Exg homologues from filamentous fungi. A recombinant protein (ExgS) has been recovered from the cultural filtrate of an Aspergillus oryzae strain that carried an expression vector containing full length of the exgS. The N-terminal amino acid sequences of the recombinant exo-1,3-β-D-glucanase (ExgS) were identical to that of native ExgS from A. saitoi.

Original language	English
Pages (from-to)	1587-1590
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	66
Issue number	7
DOIs	https://doi.org/10.1271/bbb.66.1587
Publication status	Published - 2002 Jan 1

Keywords

Aspergillus saitoi
DNA sequence
Exo-1,3-β-D-glucanase gene (exgS)
Gene cloning
Gene expression

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Cloning and expression of the exo-β-d-1,3-glucanase gene (exgs) from aspergillu saitoi",

abstract = "A gene of exo-1,3-β-D-glucanase (exgS) was cloned from a koji mold, Aspergillus saitoi, genomic DNA using PCR. The exgS has an ORF comprising 2832 bp, which contains one intron of 45 bp, and encodes 945 amino acids. The deduced amino acid sequences showed that the ExgS has a non-homologous linker region consisting of 180 amino acids, which encompassed highly conserved regions observed in Exg homologues from filamentous fungi. A recombinant protein (ExgS) has been recovered from the cultural filtrate of an Aspergillus oryzae strain that carried an expression vector containing full length of the exgS. The N-terminal amino acid sequences of the recombinant exo-1,3-β-D-glucanase (ExgS) were identical to that of native ExgS from A. saitoi.",

keywords = "Aspergillus saitoi, DNA sequence, Exo-1,3-β-D-glucanase gene (exgS), Gene cloning, Gene expression",

author = "Ken Oda and Shin Kasahara and Youhei Yamagata and Keietsu Abe and Tasuku Nakajima",

year = "2002",

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T1 - Cloning and expression of the exo-β-d-1,3-glucanase gene (exgs) from aspergillu saitoi

AU - Oda, Ken

AU - Kasahara, Shin

AU - Yamagata, Youhei

AU - Abe, Keietsu

AU - Nakajima, Tasuku

PY - 2002/1/1

Y1 - 2002/1/1

N2 - A gene of exo-1,3-β-D-glucanase (exgS) was cloned from a koji mold, Aspergillus saitoi, genomic DNA using PCR. The exgS has an ORF comprising 2832 bp, which contains one intron of 45 bp, and encodes 945 amino acids. The deduced amino acid sequences showed that the ExgS has a non-homologous linker region consisting of 180 amino acids, which encompassed highly conserved regions observed in Exg homologues from filamentous fungi. A recombinant protein (ExgS) has been recovered from the cultural filtrate of an Aspergillus oryzae strain that carried an expression vector containing full length of the exgS. The N-terminal amino acid sequences of the recombinant exo-1,3-β-D-glucanase (ExgS) were identical to that of native ExgS from A. saitoi.

AB - A gene of exo-1,3-β-D-glucanase (exgS) was cloned from a koji mold, Aspergillus saitoi, genomic DNA using PCR. The exgS has an ORF comprising 2832 bp, which contains one intron of 45 bp, and encodes 945 amino acids. The deduced amino acid sequences showed that the ExgS has a non-homologous linker region consisting of 180 amino acids, which encompassed highly conserved regions observed in Exg homologues from filamentous fungi. A recombinant protein (ExgS) has been recovered from the cultural filtrate of an Aspergillus oryzae strain that carried an expression vector containing full length of the exgS. The N-terminal amino acid sequences of the recombinant exo-1,3-β-D-glucanase (ExgS) were identical to that of native ExgS from A. saitoi.

KW - Aspergillus saitoi

KW - DNA sequence

KW - Exo-1,3-β-D-glucanase gene (exgS)

KW - Gene cloning

KW - Gene expression

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