Cloning and expression of the exo-β-d-1,3-glucanase gene (exgs) from aspergillu saitoi

Ken Oda, Shin Kasahara, Youhei Yamagata, Keietsu Abe, Tasuku Nakajima

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

A gene of exo-1,3-β-D-glucanase (exgS) was cloned from a koji mold, Aspergillus saitoi, genomic DNA using PCR. The exgS has an ORF comprising 2832 bp, which contains one intron of 45 bp, and encodes 945 amino acids. The deduced amino acid sequences showed that the ExgS has a non-homologous linker region consisting of 180 amino acids, which encompassed highly conserved regions observed in Exg homologues from filamentous fungi. A recombinant protein (ExgS) has been recovered from the cultural filtrate of an Aspergillus oryzae strain that carried an expression vector containing full length of the exgS. The N-terminal amino acid sequences of the recombinant exo-1,3-β-D-glucanase (ExgS) were identical to that of native ExgS from A. saitoi.

Original languageEnglish
Pages (from-to)1587-1590
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number7
DOIs
Publication statusPublished - 2002 Jan 1

Keywords

  • Aspergillus saitoi
  • DNA sequence
  • Exo-1,3-β-D-glucanase gene (exgS)
  • Gene cloning
  • Gene expression

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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