Cloning and expression of human B cell-specific transcription factor BACH2 mapped to chromosome 6q15

Shinya Sasaki, Etsuro Ito, Tsutomu Toki, Taira Maekawa, Rika Kanezaki, Takamichi Umenai, Akihiko Muto, Hirokazu Nagai, Tomohiro Kinoshita, Masayuki Yamamoto, Johji Inazawa, Makoto M. Taketo, Tatsutoshi Nakahata, Kazuhiko Igarashi, Masaru Yokoyama

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

The transcription factor Bach2, a member of the BTB-basic region leucine zipper (bZip) factor family, binds to a 12-O-tetradecanoylphorbol-13-acetate (TPA)-responsive element and the related Maf-recognition element (MARE) by forming homodimers or heterodimers with Maf-related transcription factors. Bach2 regulates transcription by binding to these elements. To understand the function in hematopoiesis, we isolated a cDNA clone for human Bach2 (BACH2) encoding a protein of 841 amino acid residues with a deduced amino acid sequence having 89.5% identity to mouse homolog. Among human hematopoietic cell lines, BACH2 is expressed abundantly only in some B-lymphocytic cell lines. RT-PCR analysis of hematopoietic cells revealed that BACH2 mRNA is expressed in primary B-cells. Enforced expression of BACH2 in a human Burkitt cell line, RAJI that does not express endogenous BACH2, resulted in marked reduction of clonogenic activity, indicating that BACH2 possesses an inhibitory effect on cell proliferation. By fluorescent in situ hybridization, the BACH2 gene was localized to chromosome 6q15. Because deletion of the long arm of chromosome 6 (6q) is one of the commonest chromosomal alterations in human B-cell lymphoma, we examined for the loss of heterozygosity (LOH) of the BACH2 gene in human B-cell non-Hodgkin's lymphomas (NHL). Among 25 informative cases, five (20%) showed LOH. These results indicate that BACH2 plays important roles in regulation of B cell development.

Original languageEnglish
Pages (from-to)3739-3749
Number of pages11
JournalOncogene
Volume19
Issue number33
DOIs
Publication statusPublished - 2000 Aug 3

Keywords

  • Bach2
  • CNC family
  • Maf
  • NF-E2
  • Non-Hodgkin's lymphoma

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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