Abstract
A gene (neg1) encoding an endo-1,6-β-D-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-1,6-β-D-glucanase protein of 463 amino acids and signal peptide of 17 amino acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed 1,6-β-D-glucanase activity. No genes similar in sequence were found in yeasts and fungi.
| Original language | English |
|---|---|
| Pages (from-to) | 1378-1381 |
| Number of pages | 4 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 66 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2002 Jan 1 |
Keywords
- Fungal cell wall
- Fungal β-1,6-glucanase
- Neurospora crassa
- Recombinant β-1,6-glucanase
- β-1,6-glucanase gene
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry