Cloning and expression of an Endo-1,6-β-D-glucanase gene (neg1) from neurospora crassa

Seiji Oyama, Youhei Yamagata, Keietsu Abe, Tasuku Nakajima

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)


    A gene (neg1) encoding an endo-1,6-β-D-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-1,6-β-D-glucanase protein of 463 amino acids and signal peptide of 17 amino acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed 1,6-β-D-glucanase activity. No genes similar in sequence were found in yeasts and fungi.

    Original languageEnglish
    Pages (from-to)1378-1381
    Number of pages4
    JournalBioscience, Biotechnology and Biochemistry
    Issue number6
    Publication statusPublished - 2002 Jan 1


    • Fungal cell wall
    • Fungal β-1,6-glucanase
    • Neurospora crassa
    • Recombinant β-1,6-glucanase
    • β-1,6-glucanase gene

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry


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