Cloning and expression of an Endo-1,6-β-D-glucanase gene (neg1) from neurospora crassa

Seiji Oyama, Youhei Yamagata, Keietsu Abe, Tasuku Nakajima

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

A gene (neg1) encoding an endo-1,6-β-D-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-1,6-β-D-glucanase protein of 463 amino acids and signal peptide of 17 amino acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed 1,6-β-D-glucanase activity. No genes similar in sequence were found in yeasts and fungi.

Original languageEnglish
Pages (from-to)1378-1381
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number6
DOIs
Publication statusPublished - 2002 Jan 1

Keywords

  • Fungal cell wall
  • Fungal β-1,6-glucanase
  • Neurospora crassa
  • Recombinant β-1,6-glucanase
  • β-1,6-glucanase gene

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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