Cloning and expression of a cDNA encoding the laccase from schizophyllum commune

Osamu Hatamoto, Hiroshi Sekine, Eiichi Nakano, Keietsu Abe

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

We cloned and analyzed the nucleotide sequence of a cDNA that encodes polyphenol oxidase (laccase) from the white-rot basidiomycete Schizophyllum commune.The nucleotide sequence of the full-length cDNA contained a1554-base open reading frame that encoded a polypeptide of 518 amino acid residues, including a putative signal peptide of 16 residues. It contained four highly similar regions that are conserved in the deduced amino acid sequences of otherlaccases, including the region thought to be involved in copper binding.Aspergillus sojae strain 1860 (which has low protease levels) was transformed with the plasmid lacAL/pTPT, which contained the laccase gene under the control of the tannase promoter fromAspergillus oryzae. Laccase was secreted into the medium when transformants A1 and A2 were cultured in tannic acid-containing medium.

Original languageEnglish
Pages (from-to)58-64
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume63
Issue number1
DOIs
Publication statusPublished - 1999 Jan 1
Externally publishedYes

Keywords

  • Aspergillus
  • Laccase
  • Polyphenol oxidase
  • Schizophyllum commune
  • Tannase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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