Cloning and characterization of the human UDP-N-acetylglucosamine: α- 1,3-D-mannoside β-1,4-N-acetylglucosaminyltransferase IV-homologue (hGnT-IV- H) gene

Toru Furukawa, Emile M. Youssef, Toshimasa Yatsuoka, Tadaaki Yokoyama, Naohiko Makino, Hiroko Inoue, Shinichi Fukushige, Masato Hoshi, Yutaka Hayashi, Makoto Sunamura, Akira Horii

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A novel human gene determining a polypeptide product of 478 residues with an estimated molecular mass of 55 kDa, which has significant homology and structural similarity to Bos UDP-N-acetylglucosamine: α-1,3-D-mannoside β-1,4-N-acetylglucosaminyltransferase (GnT-IV), was cloned from the commonly deleted region in pancreatic cancer at 12q21. The gene is composed of at least six exons, and the last three exons cover the open reading frame. Different sized transcripts, 3.8-kb in the heart, brain, and fetal brain and 2.8-kb and 1.7-kb in the testis were observed by Northern blot analysis. By reverse transcription-polymerase chain reaction, expression was also observed in the adult brain, liver, and adrenal gland, but not in pancreas. Because of its significant homology and structural similarity to Bos GnT-IV, it is potentially the gene encoding human GnT-IV or its homologue, which had been one of two genes remaining to be cloned in the human GnT family.

Original languageEnglish
Pages (from-to)397-401
Number of pages5
JournalJournal of Human Genetics
Volume44
Issue number6
DOIs
Publication statusPublished - 1999 Jan 1

Keywords

  • Adult brain
  • Chromosomal band 12q21
  • Fetal brain
  • Heart
  • N-acetylglucosaminyltransferase
  • Testis

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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