Cloning and characterization of rat cellular nucleic acid binding protein (CNBP) cDNA

Jun Yasuda, Shoji Mashiyama, Reiko Making, Shigekazu Ohyama, Takao Sekiya, Kenshi Hayashi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We cloned and sequenced the cDNAs which code for rat cellular nucleic acid binding protein (CNBP). In-frame insertion/deletion differences were found among the clones at two sites in the open reading frame, suggesting alternative splicing of the message or the presence of multiple genes which code for this protein. The deduced amino acid sequence revealed that one rat CNBP sequence was completely identical to its human counterpart. This striking conservation, together with the fact that homologous genes have been found in various organisms including Schizosaccharomyces pombe, suggests that CNBP plays a basic biological role in eukaryotic cells. The recombinant GST-CNBP fusion protein produced in Escherichia coli bound to a G-rich single-stranded RNA and DNA in a sequence-specific manner.

Original languageEnglish
Pages (from-to)45-49
Number of pages5
JournalDNA Research
Volume2
Issue number1
DOIs
Publication statusPublished - 1995 Jan 1

Keywords

  • CCHC box
  • GST-CNBP fusion protein
  • RNA binding
  • Zinc finger

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Yasuda, J., Mashiyama, S., Making, R., Ohyama, S., Sekiya, T., & Hayashi, K. (1995). Cloning and characterization of rat cellular nucleic acid binding protein (CNBP) cDNA. DNA Research, 2(1), 45-49. https://doi.org/10.1093/dnares/2.1.45