Cloning and characterization of developmental endothelial locus-1: An embryonic endothelial cell protein that binds the αvβ3 integrin receptor

Chiaki Hidai, Thomas Zupancic, Kalyani Penta, Adel Mikhail, Masatoshi Kawana, Elena E. Quertermous, Yoshikazu Aoka, Masafumi Fukagawa, Yasuhisa Matsui, Doros Platika, Robert Auerbach, Brigid L.M. Hogan, Ralph Snodgrass, Thomas Quertermous

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

We have taken advantage of an enhancer trap event in a line of transgenic mice to identify a unique developmentally regulated endothelial cell locus (Del1). The protein encoded in this locus contains three EGF-like repeats homologous to those in Notch and related proteins, including an EGF- like repeat that contains an RGD motif, and two discoidin I-like domains. Del1 is shown to be a matrix protein and to promote adhesion of endothelial cells through interaction with the αvβ3 integrin receptor. Embryonic endothelial-like yolk sac cells expressing recombinant Del1 protein, or grown on an extracellular matrix containing Del1 protein, are inhibited from forming vascular-like structures. Expression of Dell protein in the chick chorioallantoic membrane leads to loss of vascular integrity and promotes vessel remodeling. Del1 is thus a new ligand for the αvβ3 integrin receptor and may function to regulate vascular morphogenesis or remodeling in embryonic development.

Original languageEnglish
Pages (from-to)21-33
Number of pages13
JournalGenes and Development
Volume12
Issue number1
DOIs
Publication statusPublished - 1998 Jan 1

Keywords

  • Angiogenesis
  • Cloning
  • Embryogenesis
  • Endothelial
  • Integrin
  • Vasculature

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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  • Cite this

    Hidai, C., Zupancic, T., Penta, K., Mikhail, A., Kawana, M., Quertermous, E. E., Aoka, Y., Fukagawa, M., Matsui, Y., Platika, D., Auerbach, R., Hogan, B. L. M., Snodgrass, R., & Quertermous, T. (1998). Cloning and characterization of developmental endothelial locus-1: An embryonic endothelial cell protein that binds the αvβ3 integrin receptor. Genes and Development, 12(1), 21-33. https://doi.org/10.1101/gad.12.1.21