Cloning and characterization of a third type of human α-amylase gene, AMY2B

Hideoki Yokouchi, Akira Horii, Mitsuru Emi, Naohiro Tomita, Sadayuki Doi, Michio Ogawa, Takesada Mori, Kenichi Matsubara

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

We have previously reported concerning the existence of a third type of human α-amylase gene, AMY3 [Emi et al., Gene 62 (1988) 229-235; Tomita et al., Gene 76 (1989) 11-18], which is expressed in a lung carcinoid tissue, and differs in nucleotide sequence from the two previously characterized human α-amylase genes coding for salivary and pancreatic isozymes, termed AMY1 and AMY2, respectively. Here, we rename this gene AMY2B to coincide with the designation by Gumucio et al. [Mol. Cell Biol. 8 (1988) 1197-1205] and describe its genetic properties as revealed by sequencing studies. It consists of ten major exons whose sequences are highly homologous to those of AMY1 and AMY2. Not only the exons, but also most of the introns seem to be highly conserved, as judged from physical mapping data. The AMY2B gene identified from mRNA in a lung carcinoid tissue has at least two additional untranslated exons in its 5′ region; hence the promoter lies far upstream relative to the other two AMY genes.

Original languageEnglish
Pages (from-to)281-286
Number of pages6
JournalGene
Volume90
Issue number2
DOIs
Publication statusPublished - 1990

Keywords

  • Recombinant DNA
  • amylase isozyme
  • cosmid
  • exon
  • genomic library
  • intron
  • lung carcinoid tumor
  • multi-gene family
  • processed pseudogene
  • γ-actin

ASJC Scopus subject areas

  • Genetics

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