Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans

A. Yasui, M. Takao, A. Oikawa, A. Kiener, C. T. Walsh, A. P.M. Eker

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacz of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E.coli photolyases, notwithstanding the difference in chromophore structure.

Original languageEnglish
Pages (from-to)4447-4463
Number of pages17
JournalNucleic acids research
Volume16
Issue number10
DOIs
Publication statusPublished - 1988 May 25

ASJC Scopus subject areas

  • Genetics

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