Abstract
A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacz of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E.coli photolyases, notwithstanding the difference in chromophore structure.
| Original language | English |
|---|---|
| Pages (from-to) | 4447-4463 |
| Number of pages | 17 |
| Journal | Nucleic acids research |
| Volume | 16 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 1988 May 25 |
ASJC Scopus subject areas
- Genetics
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Cite this
Yasui, A., Takao, M., Oikawa, A., Kiener, A., Walsh, C. T., & Eker, A. P. M. (1988). Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans. Nucleic acids research, 16(10), 4447-4463. https://doi.org/10.1093/nar/16.10.4447