Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans

Akira Yasui; M. Takao; A. Oikawa; A. Kiener; C. T. Walsh; A. P.M. Eker

doi:10.1093/nar/16.10.4447

Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans

Akira Yasui, M. Takao, A. Oikawa, A. Kiener, C. T. Walsh, A. P.M. Eker

Division of Aging Science

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Abstract

A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (M_r 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacz of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E.coli photolyases, notwithstanding the difference in chromophore structure.

Original language	English
Pages (from-to)	4447-4463
Number of pages	17
Journal	Nucleic acids research
Volume	16
Issue number	10
DOIs	https://doi.org/10.1093/nar/16.10.4447
Publication status	Published - 1988 May 25

ASJC Scopus subject areas

Genetics

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title = "Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans",

abstract = "A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacz of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E.coli photolyases, notwithstanding the difference in chromophore structure.",

author = "Akira Yasui and M. Takao and A. Oikawa and A. Kiener and Walsh, {C. T.} and Eker, {A. P.M.}",

note = "Funding Information: ACKNOWLEDGEMENTS We wish to express our gratitude to Dr.O. Brocades-Zaalberg for immunization of rabbits in the Medical Biological Laboratory TNO, Rijswijk, The Netherlands. We also thank Dr.J.H.J. Hoeijmakers for his helpful suggestions. This work was supported in part by a grant-in-aid for {"}specific research on molecular mechanisms of photoreception{"} (grant number 62621001) from the Ministry of Education, Science and Culture (Japan) to A.Y. .",

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doi = "10.1093/nar/16.10.4447",

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T1 - Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans

AU - Yasui, Akira

AU - Takao, M.

AU - Oikawa, A.

AU - Kiener, A.

AU - Walsh, C. T.

AU - Eker, A. P.M.

N1 - Funding Information: ACKNOWLEDGEMENTS We wish to express our gratitude to Dr.O. Brocades-Zaalberg for immunization of rabbits in the Medical Biological Laboratory TNO, Rijswijk, The Netherlands. We also thank Dr.J.H.J. Hoeijmakers for his helpful suggestions. This work was supported in part by a grant-in-aid for "specific research on molecular mechanisms of photoreception" (grant number 62621001) from the Ministry of Education, Science and Culture (Japan) to A.Y. .

PY - 1988/5/25

Y1 - 1988/5/25

N2 - A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacz of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E.coli photolyases, notwithstanding the difference in chromophore structure.

AB - A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacz of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E.coli photolyases, notwithstanding the difference in chromophore structure.

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Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans

Abstract

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