Cloning and characterization of a c-myc intron binding protein (MIBP1)

Reiko Makino, Kosuke Akiyama, Jun Yasuda, Shoji Mashiyama, Soichi Honda, Takao Sekiya, Kenshi Hayashi

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

The cDNA for a c-myc intron 1 binding protein 1 (MIBP1) in the rat was Isolated from λgt11 and λZAPII cDNA libraries. Sequencing of the cDNA clones revealed a long ORF which encoded a putative protein of 2437 amlno acid residues. This protein has two widely separated zinc finger regions, each of which carries C2H2 motifs. When expressed in E.coll as a fusion protein, part of the MIBP1 showed sequence-specific binding to the target sequence, I.e., a 9-bp sequence in the rat c-myc intron 1. MIBP1 is most likely the rat counterpart of human MHC binding proteln-2 (MBP-2/HIV-EP2), based on the 86% similarity in nucleotlde sequence and 93% similarity in amino acid sequence. Northern blotting revealed a high level of MIBP1 mRNA in the brain.

Original languageEnglish
Pages (from-to)5679-5685
Number of pages7
JournalNucleic acids research
Volume22
Issue number25
DOIs
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Genetics

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