TY - JOUR
T1 - Cloning and characterization of a c-myc intron binding protein (MIBP1)
AU - Makino, Reiko
AU - Akiyama, Kosuke
AU - Yasuda, Jun
AU - Mashiyama, Shoji
AU - Honda, Soichi
AU - Sekiya, Takao
AU - Hayashi, Kenshi
N1 - Funding Information:
We thank the Human Genome Center, Institute of Medical Science, The University of Tokyo for computer analyses of sequences. This work was supported in part by Grants-in-Aid from the Ministry of Health and Welfare of Japan for the Comprehensive 10-Year Strategy for Cancer Control and from the Ministry of Science, Education and Culture of Japan.
PY - 1994
Y1 - 1994
N2 - The cDNA for a c-myc intron 1 binding protein 1 (MIBP1) in the rat was Isolated from λgt11 and λZAPII cDNA libraries. Sequencing of the cDNA clones revealed a long ORF which encoded a putative protein of 2437 amlno acid residues. This protein has two widely separated zinc finger regions, each of which carries C2H2 motifs. When expressed in E.coll as a fusion protein, part of the MIBP1 showed sequence-specific binding to the target sequence, I.e., a 9-bp sequence in the rat c-myc intron 1. MIBP1 is most likely the rat counterpart of human MHC binding proteln-2 (MBP-2/HIV-EP2), based on the 86% similarity in nucleotlde sequence and 93% similarity in amino acid sequence. Northern blotting revealed a high level of MIBP1 mRNA in the brain.
AB - The cDNA for a c-myc intron 1 binding protein 1 (MIBP1) in the rat was Isolated from λgt11 and λZAPII cDNA libraries. Sequencing of the cDNA clones revealed a long ORF which encoded a putative protein of 2437 amlno acid residues. This protein has two widely separated zinc finger regions, each of which carries C2H2 motifs. When expressed in E.coll as a fusion protein, part of the MIBP1 showed sequence-specific binding to the target sequence, I.e., a 9-bp sequence in the rat c-myc intron 1. MIBP1 is most likely the rat counterpart of human MHC binding proteln-2 (MBP-2/HIV-EP2), based on the 86% similarity in nucleotlde sequence and 93% similarity in amino acid sequence. Northern blotting revealed a high level of MIBP1 mRNA in the brain.
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U2 - 10.1093/nar/22.25.5679
DO - 10.1093/nar/22.25.5679
M3 - Article
C2 - 7838722
AN - SCOPUS:0028575717
VL - 22
SP - 5679
EP - 5685
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 25
ER -
