Cloning and analysis of HIV-1 envelope isolated from an infected individual in Heilongjiang province

Hai Zhou Zhou, Yan Li, Hong Ling, Yan Cheng Liu, Bing Cheng Huang, Toshio Hattori

Research output: Contribution to journalArticle

Abstract

Objective: To analyze the variability and phenotype of envelope glycoprotein(env) of human immunodeficiency virus prevalent in Heilongjiang province. Methods: Full-length env gene cloning from PBMC of a HIV-1 positive individual in Heilongjiang province, China was performed with conserved region primers. The amplified PCR products were cloned into a plasmid vector and sequenced. Phylogenetic tree analysis was done upon full length env amino acid sequence. Subsequently, a HIV-1 pseudotyped virus with the envelope protein was constructed and the infectivity was examined with U87 cell lines expressing CD4 with either CCR5 or CXCR4. Results: Two functional env clones named CHNHLJ03009c34(GenBank Accession No: AY905493) and CHNHLJ03009c33 were obtained. The homology between CHNHLJ03009c34 and a HIV-1 B′ subtype RL42 strain from Yunnan province is 91.52% through comparing and analyzing full length env amino acid sequence from domestic and abroad isolates. Phylogenetic analysis indicated that CHNHLJ03009c34 had an intimate molecular relation to strain RL42, while it became an independent arm upon the analysis of C2-V3 region. The secondary structure analysis of the envelope protein showed that the antigenicity and hydropathy of the strain did not have definite difference from RL42. Infectivity examination showed that the pseudovirus could only infect U87. CD4. CCR5 cells indicating that it comes from R5-tropic HIV-1. Conclusion: HIV-1 primary isolate envelopes CHNHLJ03009 have been cloned from a HIV-1 positive individual in Heilongjiang province. The strain is R5 Thailand B′ subtype. This is the first analysis of primary isolates in Heilongjiang.

Original languageEnglish
Pages (from-to)885-889
Number of pages5
JournalChinese Journal of Microbiology and Immunology
Volume25
Issue number11
Publication statusPublished - 2005 Nov 30

Keywords

  • Envelope
  • Human immunodeficiency virus type 1 (HIV-1)
  • Infection
  • Subtype
  • Variability

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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