Clonal structure in a dwarf bamboo (Sasa senanensis) population inferred from amplified fragment length polymorphism (AFLP) fingerprints

Yoshihisa Suyama, K. Obayashi, I. Hayashi

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Amplified fragment length polymorphism (AFLP) fingerprints were used to reveal clonal structure of a dense population of dwarf bamboo, Sasa senanensis, in a 10-ha study plot at Sugadaira Montane Research Center, University of Tsukuba, Nagano, Japan. We generated AFLP fingerprints for 51 leaf samples, collected at 50 m intervals, using three selective primer pairs. A total of 135-166 fragments were detected per sample, and 22 different fingerprints were identified based on 24-83 differing fragments. Our results demonstrate that the S. senanensis population in our plot consists of at least 22 clones and that the largest single clone occurs over a distance of about 300 m. Furthermore, the clone distribution pattern implies a relationship between site quality and clonal structure.

Original languageEnglish
Pages (from-to)901-906
Number of pages6
JournalMolecular ecology
Volume9
Issue number7
DOIs
Publication statusPublished - 2000 Aug 2

Keywords

  • AFLP
  • Bamboo
  • Clonal structure
  • Clone size
  • DNA fingerprinting
  • Sasa senanensis

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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