CLIP-170 tethers kinetochores to microtubule plus ends against poleward force by dynein for stable kinetochore-microtubule attachment

Mohammed Abdullahel Amin, Kinue Kobayashi, Kozo Tanaka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The cytoplasmic linker protein (CLIP)-170 localizes to kinetochores and is suggested to function in stable attachment of kinetochores to microtubule ends. Here we show that defects in kinetochore-microtubule attachment and chromosome alignment in CLIP-170-depleted cells were rescued by co-depletion of p150glued, a dynactin subunit required for kinetochore localization of CLIP-170. CLIP-170 recruited p150glued to microtubule ends. Kinetochore localization at microtubule ends was perturbed by CLIP-170 depletion, which was rescued by co-depleting p150glued. Our results imply that CLIP-170 tethers kinetochores to microtubule ends against the dynein-mediated poleward force to slide kinetochores along microtubules, facilitating the stable kinetochore attachment to microtubules.

Original languageEnglish
Pages (from-to)2739-2746
Number of pages8
JournalFEBS Letters
Volume589
Issue number19
DOIs
Publication statusPublished - 2015 Sep 14

Keywords

  • Chromosome alignment
  • Cytoplasmic linker protein-170
  • Dynein
  • Kinetochore
  • Microtubule
  • Mitosis

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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