CLIP-170 recruits PLK1 to kinetochores during early mitosis for chromosome alignment

Mohammed Abdullahel Amin, Go Itoh, Kenji Iemura, Masanori Ikeda, Kozo Tanaka

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

The cytoplasmic linker protein (CLIP)-170, an outer kinetochore protein, has a role in kinetochore-microtubule attachment and chromosome alignment during mitosis. However, the mechanism by which CLIP-170 is involved in chromosome alignment is not known. Here, we show that CLIP-170 colocalizes with Polo-like kinase 1 (PLK1) at kinetochores during early mitosis. Depletion of CLIP-170 results in a significant reduction in PLK1 recruitment to kinetochores and causes kinetochore-fiber (K-fiber) instability and defects in chromosome alignment at the metaphase plate. These phenotypes are dependent on the phosphorylation of CLIP-170 at a CDK1-dependent site, T287, as ectopic expression of wild-type CLIP-170, but not the expression of a non-phosphorylatable mutant, CLIP-170-T287A, restores PLK1 localization at kinetochores and rescues Kfiber stability and chromosome alignment in CLIP-170-depleted cells. These data suggest that CLIP-170 acts as a novel recruiter and spatial regulator of PLK1 at kinetochores during early mitosis, promoting K-fiber stability and chromosome alignment for error-free chromosome segregation.

Original languageEnglish
Pages (from-to)2818-2824
Number of pages7
JournalJournal of cell science
Volume127
Issue number13
DOIs
Publication statusPublished - 2014 Jul

Keywords

  • CLIP-170
  • Chromosome alignment
  • Kinetochore
  • Kinetochore-fiber
  • PLK1

ASJC Scopus subject areas

  • Cell Biology

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