Clinical studies of immunohistochemical staining of DNA-dependent protein kinase in oropharyngeal and hypopharyngeal carcinomas

K. I. Sakata, Y. Matsumoto, M. Satoh, A. Oouchi, H. Nagakura, K. Koito, Y. Hosoi, M. Hareyama, N. Suzuki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

DNA-dependent protein kinase (DNA-PK), a serine/threonine kinase composed of p470 catalytic subunit (DNA-PKcs) and p85/p70 heterodimer (Ku antigen), is considered a critical enzyme in the repair of the DNA double-strand breaks (DSB) that are the major lethal lesions induced by ionizing radiation. We investigated the expression of DNA-PK subunits in human tumors. Materials and Methods: We examined immunohistochemically the biopsy specimens of 44 patients with oropharyngeal carcinoma and 32 patients with hypopharyngeal carcinoma who had been treated with radiotherapy. Results: Immunopositivity to Ku85 and DNA-PKcs was found in all patients. The staining of Ku85 and DNA-PKcs was nuclear, with none of the normal epithelial cells or malignant cells exhibiting cytoplasmic or membrane immunoreactivity. Normal epithelial cells were all stained intensely. In tumors, intense nuclear staining of DNA-PKcs was seen in 75 of 76 tumors, while that of Ku85 was seen in all 76 patients. The radiation responses of a primary tumor that was stained weakly with DNA-PKcs were excellent. Conclusion: Our results suggest the possibility of predicting the intrinsic radiosensitivity of human tumors in clinics able to perform immunohistochemical analysis of DNA-PK.

Original languageEnglish
Pages (from-to)93-97
Number of pages5
JournalRadiation Medicine - Medical Imaging and Radiation Oncology
Volume19
Issue number2
Publication statusPublished - 2001 Jun 12
Externally publishedYes

Keywords

  • DNA-PK
  • Hypopharygeal carcinoma
  • Immunohistochemistry
  • Oropharyngeal carcinoma
  • Radiotherapy

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Oncology

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