TY - JOUR
T1 - Clinical significance of urinary interleukin-6 in children with reflux nephropathy
AU - Wang, Jun
AU - Konda, Ryuichiro
AU - Sato, Hiroshi
AU - Sakai, Kiyohide
AU - Ito, Sadayoshi
AU - Orikasa, Seiichi
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - Purpose: We determined urinary interleukin-6 (IL-6) in children with reflux nephropathy to evaluate the clinical significance of this cytokine in the progression of renal injury. Materials and Methods: Enrolled in this study were 34 boys and 32 girls in whom 99mtechnetium dimercapto-succinic acid renal scan showed renal scarring. Vesicoureteral reflux had been corrected surgically at least 3 years before study entry. Urinary IL-6 was determined by enzymelinked immunosorbent assay using spot urine samples. Simultaneously we measured serum creatinine, β2-microglobulin, α1-microglobulin, urinary α1-microglobulin and albumin. In addition, IL-6 expression was assessed by immunohistochemical study in the scarred kidneys of 3 boys and 1 girl who underwent nephrectomy due to severe reflux nephropathy with little function on renal scan. Results: Urinary IL-6 was significantly higher in children with severe bilateral renal scarring than in those with mild scarring and normal controls. Urinary IL-6 correlated significantly with serum α1-microglobulin (Spearman test p <0.03), β2-microglobulin (p <0.003), creatinine (p <0.02) and urinary albumin (p <0.0001). Histological evaluation revealed that IL-6 was predominantly expressed in the tubules in and adjacent to flbrotic areas. Conclusions: Our observations indicate that tubular IL-6 may be involved in the pathogenesis of tubulointerstitial injury in reflux nephropathy and urinary IL-6 may be a useful tool for monitoring the progression of reflux nephropathy.
AB - Purpose: We determined urinary interleukin-6 (IL-6) in children with reflux nephropathy to evaluate the clinical significance of this cytokine in the progression of renal injury. Materials and Methods: Enrolled in this study were 34 boys and 32 girls in whom 99mtechnetium dimercapto-succinic acid renal scan showed renal scarring. Vesicoureteral reflux had been corrected surgically at least 3 years before study entry. Urinary IL-6 was determined by enzymelinked immunosorbent assay using spot urine samples. Simultaneously we measured serum creatinine, β2-microglobulin, α1-microglobulin, urinary α1-microglobulin and albumin. In addition, IL-6 expression was assessed by immunohistochemical study in the scarred kidneys of 3 boys and 1 girl who underwent nephrectomy due to severe reflux nephropathy with little function on renal scan. Results: Urinary IL-6 was significantly higher in children with severe bilateral renal scarring than in those with mild scarring and normal controls. Urinary IL-6 correlated significantly with serum α1-microglobulin (Spearman test p <0.03), β2-microglobulin (p <0.003), creatinine (p <0.02) and urinary albumin (p <0.0001). Histological evaluation revealed that IL-6 was predominantly expressed in the tubules in and adjacent to flbrotic areas. Conclusions: Our observations indicate that tubular IL-6 may be involved in the pathogenesis of tubulointerstitial injury in reflux nephropathy and urinary IL-6 may be a useful tool for monitoring the progression of reflux nephropathy.
KW - Interleukin-6
KW - Kidney
KW - Kidney tubules
KW - Vesico-ureteral reflux
UR - http://www.scopus.com/inward/record.url?scp=0034751006&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034751006&partnerID=8YFLogxK
U2 - 10.1097/00005392-200101000-00060
DO - 10.1097/00005392-200101000-00060
M3 - Article
C2 - 11125408
AN - SCOPUS:0034751006
VL - 165
SP - 210
EP - 214
JO - Investigative Urology
JF - Investigative Urology
SN - 0022-5347
IS - 1
ER -