Clinical significance of methylation and reduced expression of the Quaking gene in colorectal cancer

Noriko Iwata, Toshiaki Ishikawa, Satoshi Okazaki, Kaoru Mogushi, Hironobu Baba, Megumi Ishiguro, Hirotoshi Kobayashi, Hiroshi Tanaka, Tatsuyuki Kawano, Kenichi Sugihara, Hiroyuki Uetake

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Background: This study investigated abnormal methylation in colorectal cancer (CRC) and the potential role of the Quaking RNA-binding protein (QKI) gene in tumorigenesis. Materials and Methods: Oligonucleotide microarray expression profiling was carried out on a panel of primary CRC specimens (n=17) and CRC cell lines (n=5), followed by methylation analysis using methylation-specific polymerase chain reaction. QKI expression levels were assessed in 156 primary CRCs by qRT-PCR and immunohistochemistry. Results: Low QKI expression was observed in 47.7% in CRCs. QKI promoter methylation was detected in 32.1% of patients with CRC, and in these patients mRNA expression in tumor tissue was significantly downregulated compared to matched normal tissues (p=0.049). There was a significant relationship between low QKI expression and recurrence after surgery (p=0.004). Low QKI expression was an independent risk factor for recurrence after surgery in 153 patients with CRC without distant metastases (p=0.036). Conclusion: Patients with tumors expressing low levels of QKI experienced significantly higher rates of tumor recurrence after curative surgery and worse prognoses. Methylation of the QKI promoter and concomitant reduced expression of QKI mRNA may be important for CRC initiation and progression. Loew QKI expression may be a useful clinical biomarker for predicting recurrence and prognosis.

Original languageEnglish
Pages (from-to)489-498
Number of pages10
JournalAnticancer research
Issue number2
Publication statusPublished - 2017


  • Colorectal cancer
  • Methylation
  • Prognostic factor
  • QKI
  • Quaking RNA-binding protein gene
  • Tumor-suppressor gene

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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