TY - JOUR
T1 - Clinical application of exhaled breath condensate analysis in asthma
T2 - Prediction of FEV1 improvement by steroid therapy
AU - Matsunaga, Kazuto
AU - Ichikawa, Tomohiro
AU - Yanagisawa, Satoru
AU - Akamatsu, Keiichirou
AU - Koarai, Akira
AU - Hirano, Tsunahiko
AU - Sugiura, Hisatoshi
AU - Minakata, Yoshiaki
AU - Ichinose, Masakazu
PY - 2009
Y1 - 2009
N2 - Background: Exhaled breath condensate (EBC) measurements have recently been reported to be useful for the detection of inflammatory molecules in the airways. However, the clinical relevance of EBC analysis in asthma therapy has not been determined yet. Objectives: To investigate whether EBC analysis has any potential for predicting the steroid response in asthmatics. Methods: Eighteen steroid-naive asthmatics were enrolled. EBC collection, spirometry and a methacholine challenge test were performed before and 12 weeks after inhaled steroid therapy. Exhaled IL-4, IL-17, RANTES, macrophage inflammatory protein (MIP)-1α, MIP-1β, IL-8, IFN-γ-inducible protein (IP)-10, TNF-α and TGF-β were simultaneously analyzed by a protein array, and the relationship between baseline molecule expression and steroid-mediated changes in forced expiratory volume in 1 s (FEV1) and airway responsiveness was investigated. Results: Steroid therapy improved FEV 1 values and the methacholine threshold. Among the molecules examined, increased IL-4 and RANTES levels as well as decreased IP-10 levels at baseline were significantly correlated with an improvement in FEV1. By contrast, molecule levels were not related to changes in the responsiveness to methacholine. In addition, changes in FEV1 values were significantly associated with reductions in IL-4 and RANTES levels. Conclusions: EBC measurements of IL-4, RANTES and IP-10 might be useful for predicting the steroid-mediated FEV1 improvement in asthma.
AB - Background: Exhaled breath condensate (EBC) measurements have recently been reported to be useful for the detection of inflammatory molecules in the airways. However, the clinical relevance of EBC analysis in asthma therapy has not been determined yet. Objectives: To investigate whether EBC analysis has any potential for predicting the steroid response in asthmatics. Methods: Eighteen steroid-naive asthmatics were enrolled. EBC collection, spirometry and a methacholine challenge test were performed before and 12 weeks after inhaled steroid therapy. Exhaled IL-4, IL-17, RANTES, macrophage inflammatory protein (MIP)-1α, MIP-1β, IL-8, IFN-γ-inducible protein (IP)-10, TNF-α and TGF-β were simultaneously analyzed by a protein array, and the relationship between baseline molecule expression and steroid-mediated changes in forced expiratory volume in 1 s (FEV1) and airway responsiveness was investigated. Results: Steroid therapy improved FEV 1 values and the methacholine threshold. Among the molecules examined, increased IL-4 and RANTES levels as well as decreased IP-10 levels at baseline were significantly correlated with an improvement in FEV1. By contrast, molecule levels were not related to changes in the responsiveness to methacholine. In addition, changes in FEV1 values were significantly associated with reductions in IL-4 and RANTES levels. Conclusions: EBC measurements of IL-4, RANTES and IP-10 might be useful for predicting the steroid-mediated FEV1 improvement in asthma.
KW - Airway hyperresponsiveness
KW - Airway obstruction
KW - Chemokines
KW - Cytokines
KW - Exhaled breath condensate
KW - Growth factors
KW - Steroid treatment
UR - http://www.scopus.com/inward/record.url?scp=70350340960&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70350340960&partnerID=8YFLogxK
U2 - 10.1159/000243551
DO - 10.1159/000243551
M3 - Article
C2 - 19786734
AN - SCOPUS:70350340960
VL - 78
SP - 393
EP - 398
JO - Schweizerische Zeitschrift für Tuberkulose und Pneumonologie. Revue suisse de la tuberculose et de pneumonologie. Rivista svizzera della tubercolosi e della pneumonologia
JF - Schweizerische Zeitschrift für Tuberkulose und Pneumonologie. Revue suisse de la tuberculose et de pneumonologie. Rivista svizzera della tubercolosi e della pneumonologia
SN - 0025-7931
IS - 4
ER -