Cleavage of PDGF receptor on periodontal ligament cells by elastase

E. Nemoto, S. Kanaya, M. Minamibuchi, Hidetoshi Shimauchi

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Human leukocyte elastase, a neutrophil serine protease, is considered to be a potential immunoregulatory protease. Since the PDGF receptor (PDGFR) on periodontal ligament (PDL) cells is a crucial element for various functions, such as wound healing in periodontal tissue, we investigated the effect of elastase on the expression of PDGFR on PDL cells by flow cytometry and Western blotting. We found that PDGFR-á disappeared with an increasing dose of elastase, and PDGFR-β was degraded into several fragments. Elastase degraded both receptors on fixed cells, indicating that the degradation resulted from direct proteolysis on the cell surface. Elastase also then disturbed the phosphorylation of ERK1/2, JNK/SARK, and p38, triggered by PDGF-AA and PDGF-BB, suggesting that elastase inhibited PDGFR-dependent cell activation in PDL cells. These results suggest that elastase may modulate the PDGF-mediated activity of PDL cells during periodontal wound healing.

Original languageEnglish
Pages (from-to)629-633
Number of pages5
JournalJournal of dental research
Volume84
Issue number7
DOIs
Publication statusPublished - 2005 Jul 1

Keywords

  • Elastase
  • MAP kinase
  • PDGF receptor
  • Periodontal ligament cells

ASJC Scopus subject areas

  • Dentistry(all)

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